B D P C
DNA methylation analysis platform
BISMA
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Bisulfite Sequencing DNA Methylation Analysis

Analysis of primary bisulfite sequencing DNA methylation data

CpG
Unique sequences
CpG
Repetitive sequences



BISMA software reference: BMC Bioinformatics 2010, 11:230

Reference Sequence

Submit your single sequencing files [abi] or the extracted sequences [text, fasta] either packed in one zip archive or alternatively compiled in one multi fasta file. Vector sequences will be automatically removed. Please download and inspect the example files.
Sequencing Data
Job Name [optional]
Analysis Parameter Lower threshold conversion rate %
Lower threshold sequence identity %
Upper threshold of N-sites at cytosine positions %
Upper threshold gaps allowed %
Detection of clonal molecules
Alignment
PCR-Product Sort clones by methylation
Methylation color code
Labels for nucleotides
CpG-site average Minimum number of data points necessary
Scale format
Scale Layout
Program written by Christian Rohde - 2009