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DNA methylation analysis platform
BISMA
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Bisulfite Sequencing DNA Methylation Analysis

Analysis of primary bisulfite sequencing DNA methylation data

CpG
Unique sequences
CpG
Repetitive sequences



BISMA software reference: BMC Bioinformatics 2010, 11:230

Consensus Sequence

Submit your single sequencing files [abi] or the extracted sequences [text, fasta] either packed in one zip archive or alternatively compiled in one multi fasta file. Vector sequences will be automatically removed. Please download and inspect the example files.
Sequencing Data
Job Name [optional]
Analysis Parameter Unconverted Cytosine per 100 bps
Threshold sequence identity %
Upper threshold gaps allowed %
Detection of clonal molecules
PCR-Product Sort clones by methylation
Program written by Christian Rohde - 2009


Alternative interface for direct analysis of aligned sequences in FASTA format

Analyze and plot the methylation distribution among the clones in your aligned bisulfite sequencing results

Submit your multi-fasta file. Be careful: the first sequence is treated as consensus sequence during analysis! Please download and inspect the example file.

Fasta File
Define scale in overview picture
Labels for clones
Labels for nucleotides
Sort clones by number of methylated CpG
Positioning of CpG in case of alignment gap
Size of picture
Program written by Christian Rohde - 2009